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51.
Wei W Kakizawa S Jung HY Suzuki S Tanaka M Nishigawa H Miyata S Oshima K Ugaki M Hibi T Namba S 《Phytopathology》2004,94(7):683-686
ABSTRACT Antisera raised against phloem-limited phytoplasmas generally react only with the phytoplasma strain used to produce the antigen. There is a need for an antiserum that reacts with a variety of phytoplasmas. Here, we show that an antiserum raised against the SecA membrane protein of onion yellows phytoplasma, which belongs to the aster yellows 16S-group, detected eight phytoplasma strains from four distinct 16S-groups (aster yellows, western X, rice yellow dwarf, and elm yellows). In immunoblots, approximately 96-kDa SecA protein was detected in plants infected with each of the eight phytoplasmas. Immunohistochemical staining of thin sections prepared from infected plants was localized in phloem tissues. This antiserum should be useful in the detection and histopathological analysis of a wide range of phytoplasmas. 相似文献
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Naho Yamagishi Junji Nishikawa Youichi Oshima Naoki Eguchi 《Journal of General Plant Pathology》2009,75(5):401-403
A black spot disease on cultivated alstroemeria was found in Nagano Prefecture, Japan, in January 2008. The causal fungus
isolated from the diseased plants was morphologically identified as Alternaria alstroemeriae E.G. Simmons & C.F. Hill. An inoculation test with the isolated fungus demonstrated that the disease is caused by this species.
This is the first report of black spot on alstroemeria (kokuhan-byo, in Japanese) caused by A. alstroemeriae in Japan. 相似文献
54.
Possibility of anisakid larvae infection in farmed salmon 总被引:1,自引:0,他引:1
Koji Inoue Syun-Ichiro Oshima Tatsuyoshi Hirata and Ikuo Kimura 《Fisheries Science》2000,66(6):1049-1052
SUMMARY: Nematodes belonging to the family Anisakidae including Anisakis simplex and Pseudoterranova decipiens are known to cause anisakiasis when their live larvae are ingested by humans. We estimated the possibility of anisakid infection to salmonids, farmed in sea net-pens at Onagawa Bay, Miyagi, Japan, in 1992, 1998 and 1999, by direct examination of the edible muscle and examination of the contents of the alimentary canal. From direct examination of the muscle, no nematode was found in the 249 farmed coho salmon Oncorhynchus kisutch and 40 farmed rainbow trout Oncorhynchus mykiss . In contrast, third-stage larvae of Anisakis simplex were found in seven of 14 wild coho salmon caught in Russia and all the 40 wild chum salmon Oncorhynchus keta caught at Nemuro and Kesennuma in 1998. The stomach and intestines of 521 farmed coho salmon and 40 farmed rainbow trout were examined carefully for the existence of possible carrier organisms such as crustaceans, fish or squid. Such carrier organisms were not found in the stomach and intestines of farmed fish. Thus, we conclude that the possibility of anisakid infection is very low in farmed salmonids. 相似文献
55.
Satoshi Suyama Kazuhiro Oshima Masayasu Nakagami Yasuhiro Ueno 《Fisheries Science》2009,75(2):325-333
We examined seasonal changes in the relationship between otolith radius and body length in age-zero Pacific saury Cololabis saira collected from June to December 2002–2004, based on 12,164 specimens. Results of the analysis using a generalized linear
model indicated that there were differences in the intercepts among months, but no difference was seen in the slopes. In addition,
post hoc analysis showed that the intercepts got larger as the month progressed from June to December. These results suggested
that although the body length growth rate decreased, the growth of the otolith does not decrease to the same degree as the
body after September, so the ratio of otolith radius to body length becomes larger as the months pass. Consequently, we consider
that the seasonal change in relation between otolith radius and body length affects the back-calculation of body length based
on otolith radius. 相似文献
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Yusuke Takinami Kensaku Maejima Atsushi Takahashi Takuya Keima Takuya Shiraishi Yukari Okano Ken Komatsu Kenro Oshima Shigetou Namba 《Journal of General Plant Pathology》2013,79(3):209-213
Phytoplasma-induced floral malformations such as virescence, phyllody, and proliferation were observed on hydrangeas in Gunma Prefecture, Japan. Phylogenetic analyses based on 16S rRNA, secY, groEL, and amp gene sequences indicated that the affected hydrangea plants were associated with phytoplasmas belonging to ‘Candidatus Phytoplasma asteris’, but not to ‘Ca. P. japonicum’, which occurs in hydrangeas showing phyllody in Japan. This is the first molecular evidence of an association of ‘Ca. P. asteris’ with hydrangea plants in Japan. 相似文献
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